The emergence of the brand new coronavirus 2019 (COVID-19) was first seen in December 2019, which has unfold quickly and turn into a world pandemic. The variety of instances of COVID-19 and its related mortality have raised severe issues worldwide. Early analysis of viral an infection undoubtedly permits fast intervention, illness administration, and substantial management of the fast unfold of the illness. The methodology offered right here is helpful for routine growth of tissue slices and adherent or floating cultured cells, and in addition types the idea for these variant strategies.
Currently, the usual method for COVID-19 analysis globally is the RT-qPCR take a look at; nevertheless, the restricted entry to kits and related reagents, the necessity for specialised lab tools, and the necessity for extremely expert personnel has led to a detection slowdown. Recently, the event of clustered often interspaced brief palindromic repeats (CRISPR)-based diagnostic methods has reshaped molecular analysis. The advantages of the CRISPR system resembling velocity, precision, specificity, energy, effectivity, and flexibility have impressed researchers to develop CRISPR-based diagnostic and therapeutic strategies.
With the worldwide COVID-19 outbreak, totally different teams have begun to design and develop diagnostic and therapeutic applications based mostly on the environment friendly CRISPR system. CRISPR-based COVID-19 diagnostic methods have benefits resembling a excessive detection velocity (i.e., 30 min from uncooked pattern to achieve a consequence), excessive sensitivity and precision, portability, and no want for specialised laboratory tools. Here, we assessment up to date research on the detection of COVID-19 based mostly on the CRISPR system.
A Streamlined Whole Blood CyTOF Workflow Defines A Circulating Immune Cell Signature of COVID-19
Mass cytometry (CyTOF) represents some of the highly effective instruments in immune phenotyping, permitting excessive throughput quantification of over 40 parameters at single-cell decision. However, extensive deployment of CyTOF-based immune phenotyping research are restricted by complicated experimental workflows and the necessity for specialised CyTOF tools and technical experience. Furthermore, variations in cell isolation and enrichment protocols, antibody reagent preparation, pattern staining, and information acquisition protocols can all introduce technical variation that may confound integrative analyses of enormous data-sets of samples processed throughout a number of labs.
Here, we current a streamlined entire blood CyTOF workflow which addresses many of those sources of experimental variation and facilitates wider adoption of CyTOF immune monitoring throughout websites with restricted technical experience or sample-processing sources or tools. Our workflow makes use of commercially obtainable reagents together with the Fluidigm MaxPar Direct Immune Profiling Assay (MDIPA), a dry tube 30-marker immunophenotyping panel, and SmartTube Proteomic Stabilizer, which permits for easy and dependable fixation and cryopreservation of entire blood samples.
We validate a workflow that permits for streamlined staining of entire blood samples with minimal processing necessities or experience on the web site of pattern assortment, adopted by cargo to a central CyTOF core facility for batched downstream processing and information acquisition. We apply this workflow to characterize 184 entire blood samples collected longitudinally from a cohort of 72 hospitalized COVID-19 sufferers and wholesome controls, highlighting dynamic disease-associated adjustments in circulating immune cell frequency and phenotype.
Super-resolution microscopy strategies circumvent the classical diffraction restrict of optical microscopy utilizing combos of specifically engineered excitation mild, fluorescent dyes, extremely delicate detectors, and reconstruction algorithms. Protein-retention growth microscopy (ExM) is a technique to bodily broaden organic specimens, enabling successfully sub-diffraction restricted imaging on normal microscopes with normal staining reagents. Specimen growth is pushed by a swellable gel materials that may be synthesized in situ utilizing off-the-shelf chemical substances and supplies. The growth materials and course of are strong and amenable to additional growth, which has enabled the emergence of quite a few ExM variants with prolonged capabilities from a number of unbiased labs.
Predicting the COVID-19 an infection with fourteen medical options utilizing machine studying classification algorithms
While the RT-PCR is the silver bullet take a look at for confirming the COVID-19 an infection, it’s restricted by the dearth of reagents, time-consuming, and the necessity for specialised labs. As another, many of the prior research have centered on Chest CT photographs and Chest X-Ray photographs utilizing deep studying algorithms. However, these two approaches can not at all times be used for sufferers’ screening because of the radiation doses, excessive prices, and the low variety of obtainable gadgets. Hence, there’s a want for a cheaper and sooner diagnostic mannequin to determine the constructive and detrimental instances of COVID-19.
 MOUSE SERUM:Azide Free Serum Products |
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GWB-Q00011 |
GenWay Biotech |
10 ml |
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MOUSE SERUM:Azide Free Serum Products |
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GWB-Q00012 |
GenWay Biotech |
50 ml |
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MOUSE SERUM:Azide Free Serum Products |
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GWB-Q00013 |
GenWay Biotech |
100 ml |
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MOUSE SERUM:Azide Free Serum Products |
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GWB-Q00014 |
GenWay Biotech |
500 ml |
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 RABBIT SERUM:Azide Free Serum Products |
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GWB-Q00019 |
GenWay Biotech |
20 ml |
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RABBIT SERUM:Azide Free Serum Products |
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GWB-Q00020 |
GenWay Biotech |
100 ml |
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 GUINEA PIG SERUM WITH 0.09% AZIDE Serum Products |
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GWB-Q00004 |
GenWay Biotech |
5 ml |
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 Bovine Serum Albumin Fatty Acid Free Reagent Grade |
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GWB-F06E29 |
GenWay Biotech |
1 kg |
Ask for price |
 Guinea Pig Serum |
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abx810186-10nmol |
Abbexa |
10 nmol |
EUR 650 |
Guinea Pig Serum |
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abx810186-5nmol |
Abbexa |
5 nmol |
EUR 412.5 |
 - for Serum or Plasma) Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma |
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EXOFLOW2-BASICA-SP |
SBI |
30 rxn |
EUR 1201.2 |
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 Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade |
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GWB-DCC190 |
GenWay Biotech |
1 kg |
Ask for price |
 Normal Guinea Pig Serum |
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88-NP25 |
Fitzgerald |
50 ml |
EUR 270 |
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Description: Normal Guinea Pig Serum |
Normal Guinea Pig Serum |
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88R-1016 |
Fitzgerald |
5 ml |
EUR 122 |
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Description: Normal Guinea Pig Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
 Guinea Pig Serum Albumin |
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30R-3306 |
Fitzgerald |
10 mg |
EUR 303 |
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Description: Purified native Guinea Pig serum albumin |
 Guinea pig serum albumin |
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CSB-NP002001Gp |
Cusabio |
10mg |
Ask for price |
 Guinea Pig Complement Serum |
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32R-CP004 |
Fitzgerald |
5 ml |
EUR 447 |
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Description: Purified Guinea Pig Complement adsorbed with mouse cells |
Guinea Pig Complement Serum |
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32R-CP006 |
Fitzgerald |
3 ml |
EUR 98 |
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Description: Purified Guinea Pig Complement isolated from the blood of normal Hartley guinea pigs |
Guinea Pig Complement Serum |
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32R-CP007 |
Fitzgerald |
25 ml |
EUR 807.6 |
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Description: Purified Guinea Pig Complement adsorbed with sheep red blood cells |
 Non-sterile guinea pig serum |
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GPS05-0050 |
Equitech |
50 ml |
EUR 277.68 |
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Non-sterile guinea pig serum |
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GPS05-0100 |
Equitech |
100 ml |
EUR 332.28 |
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Non-sterile guinea pig serum |
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GPS05-0500 |
Equitech |
500 ml |
EUR 667.68 |
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 Guinea Pig Serum Albumin antibody |
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70R-15101 |
Fitzgerald |
100 ug |
EUR 302 |
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|
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Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody |
 Guinea pig Serum Iron ELISA kit |
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E05S0273-192T |
BlueGene |
192 tests |
EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum Iron ELISA kit |
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E05S0273-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum Iron ELISA kit |
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E05S0273-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Guinea Pig Serum Albumin, Lyophilized |
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22070080-1 |
Glycomatrix |
50 mg |
EUR 168.11 |
Guinea Pig Serum Albumin, Lyophilized |
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22070080-2 |
Glycomatrix |
100 mg |
EUR 272.69 |
Guinea Pig Serum Albumin, Lyophilized |
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22070080-3 |
Glycomatrix |
500 mg |
EUR 728.41 |
Guinea Pig Serum Albumin, Lyophilized |
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22070080-4 |
Glycomatrix |
1000 mg |
EUR 1147.51 |
 Guinea Pig Serum Albumin ELISA kit |
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E01A42617 |
BlueGene |
96T |
EUR 700 |
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Description: ELISA |
 Guinea pig Serum amyloid ELISA kit |
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E05S0228-192T |
BlueGene |
192 tests |
EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum amyloid ELISA kit |
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E05S0228-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum amyloid ELISA kit |
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E05S0228-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Guinea pig Serum Albumin ELISA kit |
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E05S0014-192T |
BlueGene |
192 tests |
EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum Albumin ELISA kit |
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E05S0014-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum Albumin ELISA kit |
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E05S0014-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
) Guinea Pig Serum Albumin antibody (HRP) |
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60R-1144 |
Fitzgerald |
100 ug |
EUR 302 |
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Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (HRP) |
) Guinea Pig Serum Albumin antibody (FITC) |
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60R-1145 |
Fitzgerald |
100 ug |
EUR 302 |
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Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (FITC) |
) Guinea Pig Serum Albumin antibody (biotin) |
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60R-1146 |
Fitzgerald |
100 ug |
EUR 302 |
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Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (biotin) |
 Guinea Pig Serum amyloid A ELISA kit |
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E01A42729 |
BlueGene |
96T |
EUR 700 |
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Description: ELISA |
 ELISA Kit) Guinea pig Serum Albumin(BSA) ELISA Kit |
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NSL1189Gp |
Sunlong |
96T |
EUR 528 |
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 Guinea pig serum albumin lyophilized powder |
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GPSA62-0050 |
Equitech |
50mg |
EUR 460.2 |
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Guinea pig serum albumin lyophilized powder |
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GPSA62-0100 |
Equitech |
100mg |
EUR 611.52 |
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Guinea pig serum albumin lyophilized powder |
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GPSA62-0500 |
Equitech |
500mg |
EUR 1361.88 |
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Guinea pig serum albumin lyophilized powder |
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GPSA62-1000 |
Equitech |
1gm |
EUR 2388.36 |
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 Guinea Pig Serum Albumin, 10mg/mL, Sterile |
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31114007-1 |
Glycomatrix |
1 mL |
EUR 87.58 |
 Guinea Pig Serum Albumin Polyclonal Antibody |
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A57556 |
EpiGentek |
-
Ask for price
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Ask for price
-
Ask for price
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- 100 µg
- 20 ul
- 50 ul
- 100 ul
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 Mixed breed, Mouse serum -ve & G. Pig +ve) Guinea Pig control serum (non-immunized) Mixed breed, Mouse serum -ve & G. Pig +ve |
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NGPS-25-GM |
Alpha Diagnostics |
25 ml |
EUR 489.6 |
 Guinea Pig Serum amyloid A 3 ELISA kit |
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E01A42730 |
BlueGene |
96T |
EUR 700 |
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Description: ELISA |
 Guinea pig Serum amyloid A 3 ELISA kit |
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E05S0229-192T |
BlueGene |
192 tests |
EUR 1524 |
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|
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum amyloid A 3 ELISA kit |
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E05S0229-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum amyloid A 3 ELISA kit |
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E05S0229-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Guinea Pig serum albumin antibody ELISA kit |
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E01A42787 |
BlueGene |
96T |
EUR 700 |
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Description: ELISA |
 Guinea pig serum albumin antibody ELISA kit |
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E05S0304-192T |
BlueGene |
192 tests |
EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig serum albumin antibody ELISA kit |
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E05S0304-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig serum albumin antibody ELISA kit |
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E05S0304-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 Innovative Grade US Origin Guinea Pig Serum |
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IGGPSER1000ML |
Innovative research |
each |
EUR 12400 |
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Description: Innovative Grade US Origin Guinea Pig Serum |
Innovative Grade US Origin Guinea Pig Serum |
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IGGPSER100ML |
Innovative research |
each |
EUR 1395 |
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Description: Innovative Grade US Origin Guinea Pig Serum |
Innovative Grade US Origin Guinea Pig Serum |
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IGGPSER10ML |
Innovative research |
each |
EUR 163 |
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Description: Innovative Grade US Origin Guinea Pig Serum |
Innovative Grade US Origin Guinea Pig Serum |
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IGGPSER25ML |
Innovative research |
each |
EUR 388 |
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Description: Innovative Grade US Origin Guinea Pig Serum |
Innovative Grade US Origin Guinea Pig Serum |
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IGGPSER500ML |
Innovative research |
each |
EUR 6588 |
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Description: Innovative Grade US Origin Guinea Pig Serum |
Innovative Grade US Origin Guinea Pig Serum |
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IGGPSER50ML |
Innovative research |
each |
EUR 737 |
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Description: Innovative Grade US Origin Guinea Pig Serum |
 Sterile filtered guinea pig serum, 0.2 micron |
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SGP30-0050 |
Equitech |
50 ml |
EUR 193.75 |
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Sterile filtered guinea pig serum, 0.2 micron |
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SGP30-0100 |
Equitech |
100 ml |
EUR 256.25 |
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Sterile filtered guinea pig serum, 0.2 micron |
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SGP30-0500 |
Equitech |
500 ml |
EUR 775 |
|
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 ELISA Kit) Guinea pig Serum Amyloid A(SAA) ELISA Kit |
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NSL0280Gp |
Sunlong |
96T |
EUR 528 |
|
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 ELISA Kit) Guinea pig Serum Amyloid A2 (SAA2) ELISA Kit |
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abx357760-96tests |
Abbexa |
96 tests |
EUR 990 |
|
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 ELISA kit) Guinea Pig Serum response factor(SRF) ELISA kit |
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E01A42879 |
BlueGene |
96T |
EUR 700 |
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Description: ELISA |
 ELISA kit) Guinea pig Serum response factor(SRF) ELISA kit |
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E05S0401-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum response factor(SRF) ELISA kit |
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E05S0401-48 |
BlueGene |
1 plate of 48 wells |
EUR 624 |
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|
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Guinea pig Serum response factor(SRF) ELISA kit |
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E05S0401-96 |
BlueGene |
1 plate of 96 wells |
EUR 822 |
|
|
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Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
 ELISA Kit) Guinea pig Serum Response Factor (SRF) ELISA Kit |
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abx357768-96tests |
Abbexa |
96 tests |
EUR 990 |
|
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 Guinea Pig Serum Amyloid P Component ELISA kit |
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E01A42735 |
BlueGene |
96T |
EUR 700 |
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Description: ELISA |
 Guinea pig Serum Amyloid P Component ELISA kit |
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E05S0234-192T |
BlueGene |
192 tests |
EUR 1524 |
|
|
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Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Therefore, this research develops six predictive fashions for COVID-19 analysis utilizing six totally different classifiers (i.e., BayesNet, Logistic, IBk, CR, PART, and J48) based mostly on 14 medical options. This research retrospected 114 instances from the Taizhou hospital of Zhejiang Province in China. The outcomes confirmed that the CR meta-classifier is probably the most correct classifier for predicting the constructive and detrimental COVID-19 instances with an accuracy of 84.21%. The outcomes might assist in the early analysis of COVID-19, particularly when the RT-PCR kits are usually not enough for testing the an infection and help nations, particularly the creating ones that endure from the scarcity of RT-PCR checks and specialised laboratories.
