Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

The present CoVid-19 disaster is revealing the strengths and the weaknesses of the world’s capability to answer a world well being disaster. A important weak spot has resulted from the extreme centralization of the present biomanufacturing capacities, a matter of nice concern, if not a supply of nationalistic tensions. On the optimistic facet, scientific knowledge and data have been shared at an unprecedented pace fuelled by the preprint phenomena, and this has significantly strengthened our capability to develop new technology-based options.

In this work, we discover how, in a context of fast change of scientific info, plant biofactories can function a fast and simply adaptable answer for native manufacturing of bioreagents, extra particularly recombinant antibodies. For this objective, we examined our capability to provide, in the framework of an educational lab and in a matter of weeks, milligram quantities of six totally different recombinant monoclonal antibodies in opposition to SARS-CoV-2 in Nicotiana benthamiana.

For the design of the antibodies, we took benefit, amongst different knowledge sources, of the DNA sequence info made quickly available by different teams in preprint publications. mAbs have been engineered as single-chain fragments fused to a human gamma Fc and transiently expressed utilizing a viral vector. In parallel, we additionally produced the recombinant SARS-CoV-2 N protein and the receptor binding area (RBD) of the Spike protein in planta and used them to check the binding specificity of the recombinant mAbs. Finally, for two of the antibodies, we assayed a easy scale-up manufacturing protocol based mostly on the extraction of apoplastic fluid.

An environmentally-benign flow-batch system for headspace single-drop microextraction and on-drop conductometric detecting ammonium

This work presents a lab-made automated flow-batch system for headspace single-drop microextraction and on-drop conductometric sensing ammonium. Sample and NaOH answer are concurrently pumped right into a response chamber (RC), the place ammonium is transformed to ammonia by elevating pH. The transformed ammonia then diffuses into the headspace of the RC, and reacts with a 100 mM boric acid drop. The conductivity of the drop is measured by an on-drop conductivity probe, which is made by two stainless-steel contacting electrodes.

The end result exhibits that the growing charge of conductivity has a linear relationship to the ammonium focus in pattern (R2 = 0.9945). This methodology has a linear vary as much as 400 μM, a restrict of detection 2.eight μM, a relative normal deviation of 3.0% (200 μM, n = 10) and carryover coefficient 0.028. Measurements of river waters, lake waters and wastewaters have been demonstrated. The recoveries have achieved from 99.Zero to 114%. This methodology avoids utilizing of dangerous or odorous reagents and follows the idea of inexperienced chemistry.

The worldwide COVID-19 pandemic outburst has triggered a severe public well being problem with growing wants of correct and fast diagnostic and screening testing. This scenario requires an optimized administration of the chemical reagents, the consumables, and the human assets, in order to reply precisely and successfully, controlling the unfold of the illness. Testing on pooled samples maximizes the quantity of examined samples, by minimizing the time and the lab provides wanted. The normal conceptualization of the pooling methodology is predicated on mixing samples collectively in a batch.

Individual testing is required provided that a particular pool displays a optimistic end result. The growth of various hybrid strategies, based mostly on “in home” protocols, using commercially available consumables, in mixture with a dependable pooling methodology would supply an answer, specializing in the higher exploitation of the personnel and the lab provides, permitting for fast screening of a inhabitants in a fairly brief time.

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Development of novel lab-on-a-chip platform for high-throughput radioimmunoassay

Radioimmunoassay (RIA) is an especially particular and a extremely delicate sort of immunoassay, however the lengthy incubation time and technology of radioactive wastes restrict the use of RIA. To complement these disadvantages of RIA, we advise a sophisticated sort of RIA based mostly on a lab-on-a-chip (LOC) platform: μ-RIA. We designed a microfluidic chip for RIA and optimized the procedures of μ-RIA evaluation, together with floor modification, immunoreaction time, and washing. Based on the optimized situations, we performed a radioimmunoassay on the μ-RIA platform utilizing a business RIA package.

With the μ-RIA, 5 min are sufficient for evaluation. The quantity of reagent consumption is considerably diminished in contrast with standard RIA. The normal curve with R2 = 0.9951 exhibits that we are able to quantitatively consider the quantity of antigen current in unknown samples. We present the applicability of μ-RIA for the evaluation of biomolecules and the potential of μ-RIA to be a novel platform for high-throughput evaluation.

OOSA10426-20ML - SERUM:Azide Free Serum & Cells

OOSA10426-20ML 20ml
EUR 99

OOSA10430-10ML - SERUM:Azide Free Serum & Cells

OOSA10430-10ML 10ml
EUR 379

OOSA10419-100ML - SERUM:Azide Free Serum & Cells

OOSA10419-100ML 100ml
EUR 749

OOSA11794-100ML - SERUM:Azide free Serum & Cells

OOSA11794-100ML 100ml
EUR 1489

OOSA10413-5ML - Serum:Azide Free

OOSA10413-5ML 5ml
EUR 69

OOSA10415-10ML - SERUM:Azide Free

OOSA10415-10ML 10ml
EUR 139

OOSA10428-100ML - SERUM:Azide Free

OOSA10428-100ML 100ml
EUR 139

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

EXOFLOW2-BASICA-SP 30 rxn
EUR 1201.2

Free Chlorine Reagents 0-5mg/L

WAT1028 EACH
EUR 44.4

VEX Exosome Isolation Reagent (from serum)

R602 10 ml
EUR 769.2

EZ-DNA Reagents

BS8202 100preps
EUR 105.67

EZ-DNA Reagents

SK8201 100preps
EUR 112.2

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-100 each
EUR 783.6

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-25 each
EUR 294

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-5 each
EUR 144

Albumin Depletion Reagent for Plasma and Serum (20 ml)

P5W13 - Ask for price

Ecl Detection Reagents

RPN2105 EACH
EUR 724.8

Ecl Detection Reagents

RPN3004 EACH
EUR 582

OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade

OPEF01351-100G 100g
EUR 1302

MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)

WA-013 each
EUR 135

REAGENTS HIGH RANGE AMMONIA

HI73325 PK25
EUR 49.2

High Range Ammonia Reagents

HI9373301 EACH
EUR 116.4

Human IgG (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-1 1 mg
EUR 196.8

CryoProtX Mix Reagents 1.5 mL

M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Alkphos Direct Labelling Reagents

RPN3680 EACH
EUR 813.6

Set of 10 Biolipidure Reagents

Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Steroid Free Serum

90R-1007 50 mL
EUR 470.4
Description: Steroid Free Human Serum

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-BTN 100 ug
EUR 343.2

CryoProtX Mix Eco Reagents 1.5 mL

M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

PeliKine tool set, additional reagents

M1980 1 unit
EUR 177.88

OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10429-10ML 10ml
EUR 279

OOSA10433-100ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10433-100ML 100ml
EUR 1799

Human Serum (CRP free)

90R-100 100 ml
EUR 1326
Description: C-reactive protein free normal human serum

Human Serum (FABP free)

90R-109 100 ml
EUR 1326
Description: FABP free normal human serum

Bradford(Coomassie) Protein Assay Plus Reagents

P7201-050 450ml
EUR 225.6

HEK 293 Media, Serum Free

TBS8084-1L 1L
EUR 98

Human Serum (Myoglobin Free)

90R-110 100 ml
EUR 1326
Description: Myoglobin free normal human serum

OOMA00062-50ML - CRP Free Serum

OOMA00062-50ML 50ML
EUR 1379

Serum Free Medium For Rat Neural Stem Cells

RAXNF-90011 100mL
EUR 289

Human Serum (Troponin I Free)

90R-106 100 ml
EUR 1417.2
Description: Troponion I free normal human serum

Serum Free Cryopreservation Medium

TM026 25 ml
EUR 50

500mL INSECTAGRO SF9 serum free

13-410-CV PK6
EUR 222

Bovine Serum Albumin, RNase Free

40200064-1 1 mL
EUR 33.37
Description: BSA

Serum Free Complete Medium For Rat Fetal Neuron

RAXFN-90011 100mL
EUR 250

Serum Albumin, Lipid Free Protein

20-abx260049
  • EUR 927.60
  • EUR 343.20
  • EUR 627.60
  • 100 mg
  • 10 mg
  • 50 mg

Bovine Serum Albumin, protease free

GK4012-1KG 1 kg
EUR 1165.2

Bovine Serum Albumin, protease free

GK4012-500G 500 g
EUR 638.4

Albumin, Bovine Serum, Globulin Free

01281-26 100G
EUR 265.3

Albumin, Bovine Serum, Globulin Free

01281-84 50G
EUR 153.3

Albumin, Bovine Serum, Globulin Free

01281-97 10G
EUR 43.4

Bovine Serum Albumin, protease free

GK4012-1 1
EUR 2065.2

Bovine Serum Albumin, protease free

GK4012-500 500
EUR 1181.9

OOSA10412-5ML - SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10412-5ML 5ml
EUR 69

OOSA10414-10ML - SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10414-10ML 10ml
EUR 149

Purified rat serum albumin protein (RSA, >99%, globulin free)

ALBR13-N-10 10 mg
EUR 343.2

Bovine Serum Albumin, fatty acid free

GX5685-1KG 1 kg
EUR 1442.4

Bovine Serum Albumin, fatty acid free

GX5685-500G 500 g
EUR 792

Bovine Serum Albumin, fatty acid free

GX5685-1 1
EUR 1162.8

Bovine Serum Albumin, fatty acid free

GX5685-500 500
EUR 624.9

Freezing Medium (Serum-free & animal origin-free)

PB180438-100mL 100 mL
EUR 118
Description: Supplements & Reagents

Freezing Medium (Serum-free & animal origin-free)

PB180438-50mL 50 mL
EUR 65
Description: Supplements & Reagents

Normal Bovine Serum (Protease/IgG free)

88R-1012 10 ml
EUR 218.4
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Albumin, Bovine Serum, Protease Free, pH5.2

01862-74 10G
EUR 53.9

Albumin, Bovine Serum, Protease Free, pH5.2

01862-87 100G
EUR 178.5

Human Apolipoprotein AI / B Free Serum Protein

abx060869-100ml 100 ml
EUR 594

Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane

RPN2106 EACH
EUR 374.4

Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane

RPN2109 EACH
EUR 188.4

Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane

RPN2134 1KIT
EUR 572.4

Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane

RPN2209 EACH
EUR 291.6

OPMA04391-50ML - Troponin I Free Serum Protein

OPMA04391-50ML 50ML
EUR 1299

Albumin, Bovine Serum, Fatty Acid Free, pH7.0

08587-26 10G
EUR 63.7

Albumin, Bovine Serum, Fatty Acid Free, pH7.0

08587-42 25G
EUR 137.9

Albumin, Bovine Serum, Fatty Acid Free, pH7.0

08587-84 50G
EUR 184.1

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 5nm

22716-100 100ml
EUR 932
Description: 7732-18-5

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 10nm

22717-100 100ml
EUR 919
Description: 7732-18-5

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 15nm

22718-100 100ml
EUR 920
Description: 7732-18-5

ImmunoGold labeling reagents for (SEM) - Unconjugated gold colloid (GC) 20nm

22719-100 100ml
EUR 932

Corning Lymphocyte Serum Free Medium KBM551

88-551-CM 1L
EUR 201.6

Corning Lymphocyte Serum Free Medium KBM581

88-581-CM 1L
EUR 266.4

Fetal Bovine Serum, Premium Tetracyclin Free

F0500-050 500ml
EUR 1388.4

Fetal Bovine Serum (EU Origin). Tetracycline free - 100ml

FB-1280T/100 100ml
EUR 56.82

Fetal Bovine Serum (EU Origin). Tetracycline free - 500ml

FB-1280T/500 500ml
EUR 220

Serum Free Medium For Mouse Neural Stem Cells

MUXNF-90011 100mL
EUR 289

Bovine Serum Albumin Protease Free Lyophilised - kg

PM-T1726/1000 kg
EUR 549.29

Fetal Bovine Serum (South America). Tetracycline free - 100ml

FB-1001T/100 100ml
EUR 56.82

Fetal Bovine Serum (South America). Tetracycline free - 500ml

FB-1001T/500 500ml
EUR 220

Bovine Serum Albumin Protease Free Lyophilised - 100g

PM-T1726/100 100g
EUR 111.21

Bovine Serum Albumin Protease Free Lyophilised - 500g

PM-T1726/500 500g
EUR 339.79

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-1 5 g
EUR 22.36
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-2 10 g
EUR 27.79
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-3 25 g
EUR 51.82
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-4 50 g
EUR 78.47
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-5 100 g
EUR 116.35
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-6 250 g
EUR 206.54
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-7 500 g
EUR 358.96
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-8 1 kg
EUR 583.54
Description: BSA

Goat Serum (10%) in PBST with Azide

30611260-1 100 ml
EUR 33.41

Goat Serum (10%) in PBST with Azide

30611260-2 250 ml
EUR 59.36

Purified rat serum albumin protein (RSA, >99%, fatty acid and globulin free)

ALBR14-N-10 10 mg
EUR 416.4

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 724

Horse Serum (10%) in PBST with Azide

30611278-1 100 ml
EUR 31.76

Horse Serum (10%) in PBST with Azide

30611278-2 250 ml
EUR 59.79

Fetal Bovine Serum (EU Origin). Tetracycline free - 50ml

FB-1280T/50 50ml
EUR 45.49

Bovine Serum Albumin Fatty Acids Free Lyophilised - kg

PM-T1727/1000 kg
EUR 1136.69

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-100 each
EUR 783.6

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-25 each
EUR 294

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-5 each
EUR 144

Serum Free Medium For Human Fetal Neural Stem Cells

HUXNF-90011 100mL
EUR 289

Bovine Serum Albumin Fatty Acids Free Lyophilised - 100g

PM-T1727/100 100g
EUR 155.21

Bovine Serum Albumin Fatty Acids Free Lyophilised - 500g

PM-T1727/500 500g
EUR 574.42

HSA Human Serum Albumin Recombinant Protein, Lipid Free

PROTP02768-3 Regular: 50mg
EUR 565.2
Description: HSA Human Recombinant lipid reduced produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa.

SuperKine™ Serum/Protein-Free Cell Freezing Medium

BMU108-EN-100mL 100 mL
EUR 109
Description: SuperKine™ Serum/Protein-Free Cell Freezing Medium is a kind of non programmed, ready-to-use cell freezing medium with clear formula, serum-free and protein-free, which is applicable to most mammalian cells. It can eliminate the potential pathogen contamination or immune reaction risk of serum or protein, so it is safer to use, no need for programmed cooling, and can keep the recovery survival rate of various cells above 95%.

Horse Serum (10%) in PBS with Azide, pH 7.4

40120170-1 100 ml
EUR 31.76

Horse Serum (10%) in PBS with Azide, pH 7.4

40120170-2 250 ml
EUR 54.54

Fetal Bovine Serum (South America). Tetracycline free - 50ml

FB-1001T/50 50ml
EUR 45.49

Free Chlorine Reagent set (25 tests)

HI-701-25 PK25
EUR 10.8

Xpro IP Cell Lysis Reagent,SDS Free

X3100-010 100ml
EUR 208.8

Xpro IP Cell Lysis Reagent,SDS Free

X3100-050 500ml
EUR 380.4

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-100 each
EUR 1358.4

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-25 each
EUR 535.2

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-5 each
EUR 210

cAMP Biotrak(TM) EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251

GERPN2251 EACH
EUR 685.2

30(w/v)%-Albumin, Bovine Serum, Solution, Fatty Acid Free

19361-84 20ML
EUR 105

Goat Serum (10%) in TBST Buffer with Azide

30611263-1 100 mL
EUR 33.41

Goat Serum (10%) in TBST Buffer with Azide

30611263-2 250 ml
EUR 57.19

EP Reagent Carbon Dioxide Free Water

1095502 1L
EUR 76.8

EP Reagent Carbon Dioxide Free Water

1095502TO 1L
EUR 124.03

Serum Free Medium (Type II) For Mouse Embryonic Stem Cells

MUXES-90061 200mL
EUR 839

Serum Free Medium (Type I) For Mouse Embryonic Stem Cells

MUXES-90062 200mL
EUR 839

Human Serum Albumin Recombinant Protein Tag Free Lyophilized

IHUSERALBRTFLY50UG each
EUR 398
Description: Human Serum Albumin Recombinant Protein Tag Free Lyophilized

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-100 each
EUR 1096.8

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-1000 each Ask for price

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-25 each
EUR 444

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-5 each
EUR 183.6

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Tissue Culture Media

EXOFLOW2-BASICA-TC 30 rxn
EUR 1201.2

Serum-Free 293 Media

TM099 500ml
EUR 50

Human Lambda Light Chain (free) goat polyclonal antibody, Serum

AP31533SU-N 1 ml Ask for price

Goat Serum (10%) in TBST Wash Buffer with Azide

30611266-1 100 ml
EUR 33.41

Goat Serum (10%) in TBST Wash Buffer with Azide

30611266-2 250 ml
EUR 57.11

Serum-Free CHO Media

TM098 500ml
EUR 50

Rat Serum

abx098267-10ml 10 ml
EUR 326.4

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)

NSPF-1 1 ml
EUR 343.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)

NSPF-10 10 ml
EUR 781.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)

NSPF-5 5 ml
EUR 634.8

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0

7907-100 each
EUR 783.6

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0

7907-25 each
EUR 294

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0

7907-5 each
EUR 144

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2

7908-100 each
EUR 783.6

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2

7908-25 each
EUR 294

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2

7908-5 each
EUR 144

HaCaT Cells (Serum-Free)

T0020001.1 One Frozen vial
EUR 570

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile

30611251-1 100 ml
EUR 32.94

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile

30611251-2 250 ml
EUR 59.44

Donkey Serum (10%) in PBST and Azide, pH 7.4, Sterile

30611269-1 50 mL
EUR 36.77

New platforms are enabling radiochemistry to be carried out in tiny, microliter-scale volumes, and this functionality has monumental advantages for the manufacturing of radiopharmaceuticals. These droplet-based applied sciences can obtain comparable or higher yields in comparison with standard strategies, however with vastly diminished reagent consumption, shorter synthesis time, larger molar exercise (even for low exercise batches), quicker purification, and ultra-compact system dimension. We evaluate right here the state of the artwork of this rising route, summarize the radiotracers and prosthetic teams which were synthesized in droplet format, describe latest achievements in scaling up exercise ranges, and talk about benefits and limitations and the long run outlook of these modern gadgets.