Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

The present CoVid-19 disaster is revealing the strengths and the weaknesses of the world’s capability to answer a world well being disaster. A important weak spot has resulted from the extreme centralization of the present biomanufacturing capacities, a matter of nice concern, if not a supply of nationalistic tensions. On the optimistic facet, scientific knowledge and data have been shared at an unprecedented pace fuelled by the preprint phenomena, and this has significantly strengthened our capability to develop new technology-based options.

In this work, we discover how, in a context of fast change of scientific info, plant biofactories can function a fast and simply adaptable answer for native manufacturing of bioreagents, extra particularly recombinant antibodies. For this objective, we examined our capability to provide, in the framework of an educational lab and in a matter of weeks, milligram quantities of six totally different recombinant monoclonal antibodies in opposition to SARS-CoV-2 in Nicotiana benthamiana.

For the design of the antibodies, we took benefit, amongst different knowledge sources, of the DNA sequence info made quickly available by different teams in preprint publications. mAbs have been engineered as single-chain fragments fused to a human gamma Fc and transiently expressed utilizing a viral vector. In parallel, we additionally produced the recombinant SARS-CoV-2 N protein and the receptor binding area (RBD) of the Spike protein in planta and used them to check the binding specificity of the recombinant mAbs. Finally, for two of the antibodies, we assayed a easy scale-up manufacturing protocol based mostly on the extraction of apoplastic fluid.

An environmentally-benign flow-batch system for headspace single-drop microextraction and on-drop conductometric detecting ammonium

This work presents a lab-made automated flow-batch system for headspace single-drop microextraction and on-drop conductometric sensing ammonium. Sample and NaOH answer are concurrently pumped right into a response chamber (RC), the place ammonium is transformed to ammonia by elevating pH. The transformed ammonia then diffuses into the headspace of the RC, and reacts with a 100 mM boric acid drop. The conductivity of the drop is measured by an on-drop conductivity probe, which is made by two stainless-steel contacting electrodes.

The end result exhibits that the growing charge of conductivity has a linear relationship to the ammonium focus in pattern (R2 = 0.9945). This methodology has a linear vary as much as 400 μM, a restrict of detection 2.eight μM, a relative normal deviation of 3.0% (200 μM, n = 10) and carryover coefficient 0.028. Measurements of river waters, lake waters and wastewaters have been demonstrated. The recoveries have achieved from 99.Zero to 114%. This methodology avoids utilizing of dangerous or odorous reagents and follows the idea of inexperienced chemistry.

The worldwide COVID-19 pandemic outburst has triggered a severe public well being problem with growing wants of correct and fast diagnostic and screening testing. This scenario requires an optimized administration of the chemical reagents, the consumables, and the human assets, in order to reply precisely and successfully, controlling the unfold of the illness. Testing on pooled samples maximizes the quantity of examined samples, by minimizing the time and the lab provides wanted. The normal conceptualization of the pooling methodology is predicated on mixing samples collectively in a batch.

Individual testing is required provided that a particular pool displays a optimistic end result. The growth of various hybrid strategies, based mostly on “in home” protocols, using commercially available consumables, in mixture with a dependable pooling methodology would supply an answer, specializing in the higher exploitation of the personnel and the lab provides, permitting for fast screening of a inhabitants in a fairly brief time.

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Development of novel lab-on-a-chip platform for high-throughput radioimmunoassay

Radioimmunoassay (RIA) is an especially particular and a extremely delicate sort of immunoassay, however the lengthy incubation time and technology of radioactive wastes restrict the use of RIA. To complement these disadvantages of RIA, we advise a sophisticated sort of RIA based mostly on a lab-on-a-chip (LOC) platform: μ-RIA. We designed a microfluidic chip for RIA and optimized the procedures of μ-RIA evaluation, together with floor modification, immunoreaction time, and washing. Based on the optimized situations, we performed a radioimmunoassay on the μ-RIA platform utilizing a business RIA package.

With the μ-RIA, 5 min are sufficient for evaluation. The quantity of reagent consumption is considerably diminished in contrast with standard RIA. The normal curve with R2 = 0.9951 exhibits that we are able to quantitatively consider the quantity of antigen current in unknown samples. We present the applicability of μ-RIA for the evaluation of biomolecules and the potential of μ-RIA to be a novel platform for high-throughput evaluation.

Human Free PSA (f-PSA) ELISA Kit

PRB-5049-FREE 96 assays
EUR 572

INSECTAGRO SF9, SERUM FREE

13-410-CV 500 mL/pk
EUR 124
Description: Specialty Media; Insect Media Products

Human Serum (CRP free)

90R-100 100 ml
EUR 1105
Description: C-reactive protein free normal human serum

Human Serum (FABP free)

90R-109 100 ml
EUR 1105
Description: FABP free normal human serum

Human Serum (Myoglobin Free)

90R-110 100 ml
EUR 1105
Description: Myoglobin free normal human serum

Myoglobin-Free Serum Protein

abx069893-50ml 50 ml
EUR 1121
  • Shipped within 5-10 working days.

VitroPlus III, Serum-Free, Xeno-Free, Complete

PC00B2 500 ml
EUR 375

EZ-DNA Reagents

BS8202 100preps
EUR 88.06
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

EZ-DNA Reagents

SK8201 100preps
EUR 93.5
  • Product category: Molecular Biology Kits/DNA - Extraction (Genomic)/Multipurpose

Human Serum (Troponin I Free)

90R-106 100 ml
EUR 1181
Description: Troponion I free normal human serum

Serum Albumin, Lipid Free Protein

20-abx260049
  • EUR 773.00
  • EUR 286.00
  • EUR 523.00
  • 100 mg
  • 10 mg
  • 50 mg
  • Shipped within 5-10 working days.

Bovine Serum Albumin, protease free

GK4012-1KG 1 kg
EUR 971

Bovine Serum Albumin, protease free

GK4012-500G 500 g
EUR 532

PK15 Cell Serum-Free Medium

VCum-Lsx0007 1 L
EUR 758
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

CHO Cell Serum-Free Medium

VCum-Lsx0025 1L
EUR 635
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium

VCum-Lsx0027 1L
EUR 635
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium

VCum-Lsx0029 1L
EUR 682
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium

VCum-Lsx0031 1 L
EUR 758
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Human Free PSA (f-PSA) ELISA Kit

PRB-5049-FREE-5 5 x 96 assays
EUR 2283

CORNING® LYMPHOCYTE SERUM FREE MEDIUM

88-551-CM 1/pk
EUR 149
Description: Specialty Media; Kohjin Products

CORNING® LYMPHOCYTE SERUM FREE MEDIUM

88-581-CM 1/pk
EUR 189
Description: Specialty Media; Kohjin Products

Normal Bovine Serum (Protease/IgG free)

88R-1012 10 ml
EUR 182
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

B-27 Serum-Free Supplement (50x)

abx082466-10ml 10 ml
EUR 328
  • Shipped within 5-10 working days.

Bovine Serum Albumin, fatty acid free

GX5685-1KG 1 kg
EUR 1202

Bovine Serum Albumin, fatty acid free

GX5685-500G 500 g
EUR 660

Fetal Bovine Serum, Premium Tetracyclin Free

F0500-050 500ml
EUR 1157

ST Cell Serum-Free Medium-1

VCum-Lsx0001 1 L
EUR 740
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2

VCum-Lsx0003 1 L
EUR 758
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

PK15 Cell Serum-Free Medium (Powder)

VCum-Lsx0008-10L 10 L
EUR 2332
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

PK15 Cell Serum-Free Medium (Powder)

VCum-Lsx0008-1L 1 L
EUR 278
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

PK15 Cell Serum-Free Medium (Powder)

VCum-Lsx0008-5L 5 L
EUR 1191
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

MDCK Cell Serum-Free Medium-1

VCum-Lsx0009 1 L
EUR 682
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-2

VCum-Lsx0011 1 L
EUR 694
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

BHK Cell Serum-Free Medium-1

VCum-Lsx0017 1 L
EUR 647
Description: BHK cell serum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-2

VCum-Lsx0019 1 L
EUR 717
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

Insect Cell Serum-Free Medium-1

VCum-Lsx0021 1 L
EUR 740
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2

VCum-Lsx0023 1 L
EUR 758
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

CHO Cell Serum-Free Medium (Powder)

VCum-Lsx0026-10L 10 L
EUR 1747
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

CHO Cell Serum-Free Medium (Powder)

VCum-Lsx0026-1L 1 L
EUR 220
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

CHO Cell Serum-Free Medium (Powder)

VCum-Lsx0026-5L 5 L
EUR 898
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium (Powder)

VCum-Lsx0028 50 L
EUR 6411
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium (Powder)

VCum-Lsx0030 50 L
EUR 8591
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium (Powder)

VCum-Lsx0032-10L 10 L
EUR 2332
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Vero Cell Serum-Free Medium (Powder)

VCum-Lsx0032-1L 1 L
EUR 282
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Vero Cell Serum-Free Medium (Powder)

VCum-Lsx0032-5L 5 L
EUR 1208
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Purified rat serum albumin protein (RSA, >99%, globulin free)

ALBR13-N-10 10 mg
EUR 286

Set of 10 Biolipidure Reagents

Biolipidure-set 10mLx10
EUR 1517
  • Biolipidure enhances sensitivity and accuracy.
  • Biolipidure suppresses non-specific adsorption.
  • Biolipidure stabilizes antibodies and enzymes.
  • Biolipidure eliminates lot-to-lot variations.
  • Biolipidure does not require biohazardous handling.
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Human Apolipoprotein AI / B Free Serum Protein

abx060869-100ml 100 ml
EUR 495
  • Shipped within 5-10 working days.

ST Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0002-10L 10 L
EUR 2215
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0002-1L 1 L
EUR 266
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0002-5L 5 L
EUR 1132
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0004-10L 10 L
EUR 2332
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0004-1L 1 L
EUR 278
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0004-5L 5 L
EUR 1191
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

MDCK Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0010-10L 10 L
EUR 1864
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0010-1L 1 L
EUR 231
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0010-5L 5 L
EUR 957
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0012-10L 10 L
EUR 1922
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

MDCK Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0012-1L 1 L
EUR 237
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

MDCK Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0012-5L 5 L
EUR 986
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

BHK Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0018-10L 10 L
EUR 1688
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0018-1L 1 L
EUR 214
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0018-5L 5 L
EUR 869
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0020-10L 10 L
EUR 1922
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

BHK Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0020-1L 1 L
EUR 237
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

BHK Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0020-5L 5 L
EUR 986
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

Insect Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0022-10L 10 L
EUR 2215
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0022-1L 1 L
EUR 266
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-1 (Powder)

VCum-Lsx0022-5L 5 L
EUR 1132
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0024-10L 10 L
EUR 2332
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0024-1L 1 L
EUR 278
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)

VCum-Lsx0024-5L 5 L
EUR 1191
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

EXOFLOW2-BASICA-SP 30 rxn
EUR 1001
  • Category: Exosome

Bradford(Coomassie) Protein Assay Plus Reagents

P7201-050 450ml
EUR 188

Bovine serum albumin (BSA) protein (>99%, Protease-free)

BSA16-N-100 100 mg
EUR 286

Insect Cell Medium: Serum-Free Insect Culture Medium

ABP-MED-10002 1 liter Ask for price
    • Product line: Cell Culture Reagents
    • Brand:

HSA Human Serum Albumin Recombinant Protein, Lipid Free

PROTP02768-3 Regular: 50mg
EUR 471
Description: HSA Human Recombinant lipid reduced produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa.

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)

RAT-5 1
EUR 1138

Rat Serum

abx098267-10ml 10 ml
EUR 272
  • Shipped within 5-10 working days.

500 ML INSECTAGRO® DS2 SERUM-FREE/PROTEIN-FREE MEDIUM, 1X WITHOUT L-GLUTAMINE

13-402-CV 500 mL/pk
EUR 153
Description: Specialty Media; Insect Media Products

Human Male AB Serum (Converted from Plasma), Xeno-Free

AR1005-0100 100mL Ask for price

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-100
EUR 653

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-25
EUR 245

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-5
EUR 120

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)

NSPF-1 1 ml
EUR 286

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)

NSPF-10 10 ml
EUR 651

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)

NSPF-5 5 ml
EUR 529

Purified rat serum albumin protein (RSA, >99%, fatty acid and globulin free)

ALBR14-N-10 10 mg
EUR 347

Free-T3/ Rat Free- T3 ELISA Kit

ELA-E0450r 96 Tests
EUR 886

Human IgG (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-1 1 mg
EUR 164

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-100
EUR 914

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-1000 Ask for price

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-25
EUR 370

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-5
EUR 153

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-100
EUR 1132

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-25
EUR 446

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-5
EUR 175

Rat Serum Albumin

30R-3297 10 mg
EUR 187
Description: Purified native Rat Serum Albumin

Rat Complement Serum

32R-CR005 5 ml
EUR 435
Description: Purifed Rat Complement isolated from blood of normal rats

New platforms are enabling radiochemistry to be carried out in tiny, microliter-scale volumes, and this functionality has monumental advantages for the manufacturing of radiopharmaceuticals. These droplet-based applied sciences can obtain comparable or higher yields in comparison with standard strategies, however with vastly diminished reagent consumption, shorter synthesis time, larger molar exercise (even for low exercise batches), quicker purification, and ultra-compact system dimension. We evaluate right here the state of the artwork of this rising route, summarize the radiotracers and prosthetic teams which were synthesized in droplet format, describe latest achievements in scaling up exercise ranges, and talk about benefits and limitations and the long run outlook of these modern gadgets.